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FuGENE®HD |
Lipofectamine® 2000 | |
![]() Reagent / DNA Ratio 0.3μl/0.1μg 8μl Complex per well Complex Incubation 5 min |
Complexes are formed in Sterile Deionized Water | ![]() Reagent / DNA Ratio 0.5μl/0.2μg 50μl Complex per well Complex Incubation 20 min |
![]() Reagent / DNA Ratio 0.3μl/0.1μg 8μl Complex per well Complex Incubation 5 min |
Complexes are formed in OptiMEM® | ![]() Reagent / DNA Ratio 0.5μl/0.2μg 50μl Complex per well Complex Incubation 20 min |
![]() Reagent / DNA Ratio 0.3μl/0.1μg 8μl Complex per well Complex Incubation 5 min |
Complexes are formed in Growth medium DMEM+10% Fetal Bovine Serum | ![]() Reagent / DNA Ratio 0.5μl/0.2μg 50μl Complex per well Complex Incubation 20 min |
FuGENE®HD
|
Lipofectamine® 2000
|
|
Transfection with competitor reagent was performed according to manufacturer’s instructions |
MCF-7 cells were seeded from 90-100% confluent culture with the density 15000 cells/well in 100μl complete growth medium, DMEM + 10% Fetal Bovine Serum the day before transfection to reach approximately 80% confluence at the time of experiment.
Tissue culture 96-round bottom well plates were used for complex preparation:
Optimal ratio of Reagent/DNA varies from 0.25/0.1 to 0.35/0.1.
Place the cells into CO2 incubator for 48 hours.