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Transfection with competitor reagent was performed according to manufacturer’s instructions
HeLa cells were seeded from 90-100% confluent culture the day before transfection with the density 10,000 cells per well in 100 μl complete growth medium DMEM + 10% Fetal Bovine Serum.
Tissue culture 96-round bottom well plates were used for complex preparation:
Optimal ratio Reagent/DNA may vary in range 0.25μl/0.1μg to 0.35μl/0.1μg.
Place the cells into CO2 incubator for 24 hours.