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FuGENE®HD |
Optifect® |
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Reagent / DNA Ratio 0.3μl/0.1μg 5μl Complex per well Complex Incubation 5 min | Reagent / DNA Ratio 1.5μl/0.25μg 5μl Complex per well Complex Incubation 5 min | |
FuGENE®HD |
Optifect® |
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Reagent / DNA Ratio 0.2μl/0.1μg 5μl Complex per well Complex Incubation 15 min | Reagent / DNA Ratio 1μl/0.25μg 5μl Complex per well Complex Incubation 15 min | |
Transfection with competitor reagent was performed according to manufacturer’s instructions |
HeLa cells were seeded from 90-100% confluent culture the day before transfection with the density 10,000 cells per well (optional cell density as low as 1,000 and 3,000 cells per well can be used) in 100 μl complete growth medium DMEM + 10% Fetal Bovine Serum.
Tissue culture 96-round bottom well plates were used for complex preparation:
Optimal ratio of reagent/DNA varies from 0.25μl/0.1μg to 0.4μl/0.1μg.
Transfected cells were incubated in CO2 incubator for 72 hours.