Click Here To View Protocol
Reagent / DNA Ratio
8μl Complex per well
Complex Incubation 5 min
*An important carrier line for virus production
Cells were seeded in 96-well non-tissue culture plate just before transfection with the density 50,000 cells per well in 100 μl “FreeStyle™ 293” growth medium (Invitrogen, Inc.®).
Tissue culture 96-round bottom well plates were used for complex preparation:
Optimal reagent/DNA ratio may vary in the range 0.25μl/0.1μg to 0.45μl/0.1μg.
Place the cells into CO2 incubator for 72 hours.
Monitor the transgene expression under fluorescent microscope or flowcytometer.